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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been described. Two additional splice variants ended up documented but comprehensive nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide generally includes the epitope regarded from the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to be certain the very best benefits are...
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This guide outlines further achievable optimization of mobile and immunoassay parameters to make sure the best possible benefits are...
Antibodies sure to the blocking peptide no longer bind to the epitope within the focus on protein. By evaluating the staining from your blocked antibody vs . the antibody on your own, you can see which staining is distinct.
Concentrate on Info Cyclin E belongs to your hugely conserved cyclin relatives, whose customers are characterised by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation patterns which add to the temporal coordination of each and every mitotic party. Cyclin E forms a complex with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and website is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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